411-8540
静岡県三島市谷田1111
国立遺伝学研究所 系統生物研究センター
酒井則良

Noriyoshi Sakai, Genetic strains Research Center, National Institute of Genetics, 1111 Yata, Mishima 411-8540, Japan

研究実績

2004年以降

Article

  • Shibata E, Liu Z, Kawasaki T, Sakai N, Kawakami A. (2018). Robust and local positional information within a fin ray directs fin length during zebrafish regeneration. Dev Growth Differ 60, 354-364.
  • Higaki, S, Todo, T, Teshima, R, Tooyama, I, Fujioka, Y, Sakai, N, Takada, T. (2018). Cryopreservation of male and female gonial cells by vitrification in the critically endangered cyprinid honmoroko Gnathopogon caerulescens. Fish Physiol Biochem 44, 503-513.
  • Nishimura T, Yamada K, Fujimori C, Kikuchi M, Kawasaki T, Siegfried KR, Sakai N, Tanaka M. (2018). Germ cells in the teleost fish medaka have an inherent feminizing effect. PLoS genet 14, e1007259.
  • Kawasaki T, Maeno A, Shiroishi T, Sakai N. (2017). Development and growth of organs in living whole embryo and larval grafts in zebrafish. Sci Rep 7, 16508.
    （ゼブラフィッシュrag1変異体に胚または稚魚を皮下移植して成長させる方法を示した論文）
  • Higaki S, Shimada M, Kawamoto K, Todo T, Kawasaki T, Tooyama I, Fujioka Y, Sakai N, Takada T. (2017). In vitro differentiation of fertile sperm from cryopreserved spermatogonia of the endangered endemic cyprinid honmoroko ( Gnathopogon caerulescens). Sci Rep 7, 42852.
  • Kawasaki T, Sakai N. (2016). Allogeneic transplantation of testicular hyperplasia in rag1 mutant zebrafish. Bio-protocol 6, e1992.
    （ゼブラフィッシュrag1変異体に皮下移植して肥大化精巣を維持させる方法を示した論文）
  • Kawasaki T, Siegfried K R, Sakai N. (2016). Differentiation of zebrafish spermatogonial stem cells to functional sperm in culture. Development 143, 566-574.
    （精原幹細胞を皮下移植法により維持する方法と、精原幹細胞から機能的精子までの培養法を確立した論文）
  • Higaki S, Shimada M, Koyama Y, Fujioka Y, Sakai N, Takada T. (2015). Development and characterization of an embryonic cell line from endangered endemic cyprinid Honmoroko Gnathopogon caerulescens (Sauvage, 1883). In Vitro Cell Dev Biol-Animal 51, 763-768.
  • Nishimura T, Herpin A, Kimura T, Hara I, Kawasaki T, Nakamura S, Yamamoto Y, Saito T L, Yoshimura J, Morishita S, Tsukahara T, Kobayashi S, Naruse K, Shigenobu S, Sakai N, Schartl M, Tanaka M. (2014). Analysis of a novel gene, Sdgc, reveals sex chromosome-dependent differences of medaka germ cells prior to gonad formation. Development 141, 3363-3369.
  • Saito K, Sakai C, Kawasaki T, Sakai N. (2014). Telomere distribution pattern and synapsis initiation during spermatogenesis in zebrafish. Dev Dyn 243, 1448-1456.
    （ゼブラフィッシュ精母細胞においてテロメアの挙動を調べ、レプトテン期からザイゴテン期にかけて明瞭なテロメアブーケ構造ができることを示した論文）
  • Mochiduki T, Sakai N, Nakajima T, Nakajima M. (2013). The relationship between HSP70 accumulation and acquisition of thermal tolerance in the guppy, Poecilia reticulata. Fish Genet Breed Sci 42, 91-98.
  • Higaki S, Koyama Y, Shimada M, Toyoyama I, Fujioka Y, Sakai N, Ikeuchi T, Takada T. (2013). Hormone response of Sertoli cell line endogenously expressing nuclear receptors in endemic cyprinid Gnathopogon caerulescens . Genes Cells 17, 316-325.
  • Koyama Y, Higaki S, Fujioka Y, Sakai N, Takada T. (2013). Establishment of testicular and ovarian cell lines from Honmoroko (Gnathopogon caerulescens). Fish Physiol Biochem 39, 701-711.
  • Shinya M, Kobayashi K, Masuda A, Tokumoto M, Ozaki Y, Saito K, Kawasaki T, Sado Y, Sakai N. (2013). Properties of gene knockdown system by vector-based siRNA in zebrafish. Dev Growth Differ 55, 755-765.
    （ゼブラフィッシュにsiRNAを発現するDNAコンストラクトを導入して、RNAiにより遺伝子発現抑制ができることを示した論文）
  • Kawasaki T, Saito K, Sakai C, Shinya M, Sakai N (2012). Production of zebrafish offspring from cultured spermatogonial stem cells. Genes Cells 17, 316-325.
    （ゼブラフィッシュの精原幹細胞培養系を確立した論文）
  • Ozaki Y, Saito K, Shinya M, Kawasaki T, Sakai N (2011). Evaluation of Sycp3, Plzf and Cyclin B3 expression and suitability as spermatogonia and spermatocyte markers in zebrafish. Gene Expr Patterns 11, 309-315.
    （ゼブラフィッシュ精巣におけるSycp3とPlzfの発現パターンを示した論文）
  • Saito K, Siegfried KR, Nusslein-Volhard C, Sakai N (2011). Isolation and cytogenetic characterization of zebrafish meiotic prophase I mutants. Dev Dyn 240, 1779-1792.
    （ゼブラフィッシュで第一減数分裂が異常となる突然変異体を単離した論文）
  • Shinya M, Sakai N (2011). Generation of highly homogeneous strains of zebrafish through full sib-pair mating. G3 1, 377-386.
    （ゼブラフィッシュの近交系樹立の論文）
  • Torihara H, Uechi T, Chakraborty A, Shinya M, Sakai N, Kenmochi N (2011). Erythropoiesis failure due to RPS19 deficiency is independent o f an activated p53 response in a zebrafish model of Diamond-Blackfan anaemia. British J Haematol 152, 648-654.
  • Kawasaki T, Saito K, Shinya M, Olsen LC, Sakai N (2010). Regeneration of spermatogenesis and production of functional sperm by grafting of testicular cell aggregates in zebrafish. Biol Reprod 83, 533-539.
    （ゼブラフィッシュで精巣細胞再集合塊を移植して精巣構造を再構築した論文）
  • Kawasaki T, Saito K, Mitsui K, Ikawa M, Yamashita M, Taniguchi Y, Takeda S, Mitani K, Sakai N (2009). Introduction of a foreign gene into zebrafish and medaka cells using adenoviral vectors. Zebrafish 6, 253-258.
    （アデノウイルスがゼブラフィッシュ細胞にも感染することを示した論文）
  • Hashiguchi M, Shinya M, Tokumoto M, Sakai N (2008). Nodal/Bozozok-independent induction of the dorsal organizer by zebrafish cell lines. Dev Biol 321, 387-396.
    （ゼブラフィッシュ初期胚由来培養細胞が二次胚誘導能をもつことを示した論文）
  • Kimura T, Shimada A, Sakai N, Mitani H, Naruse K, Takeda H, Inoko H, Tamiya G, Shinya M (2007). Genetic analysis of craniofacial traits in the medaka. Genetics 177, 2379-2388.
  • Sakai N (2006). In vitro male germ cell cultures of zebrafish. Methods 39, 239-245.
  • Kimura T, Yoshida K, Shimada A, Jindo T, Sakaizumi M, Mitani H, Naruse K, Takeda H, Inoko H, Tamiya G, Shinya M (2005). Genetic linkage map of medaka with polymerase chain reaction length polymorphisms. Gene 363, 24-31.
  • Tamiya G, Shinya M, Imanishi T, Ikuta T, Makino S, Okamoto K, Furugaki K, Matsumoto T, Mano S, Ando S, Nozaki Y, Yukawa W, Nakashige R, Yamaguchi D, Ishibashi H, Yonekura M, Nakami Y, Takayama S, Endo T, Saruwatari T, Yagura M, Yoshikawa Y, Fujimoto K, Oka A, Chiku S, Linsen SE, Giphart MJ, Kulski JK, Fukazawa T, Hashimoto H, Kimura M, Hoshina Y, Suzuki Y, Hotta T, Mochida J, Minezaki T, Komai K, Shiozawa S, Taniguchi A, Yamanaka H, Kamatani N, Gojobori T, Bahram S, Inoko H (2005). Whole genome association study of rheumatoid arthritis using 27 039 microsatellites. Hum Mol Genet 14, 2305-2321.
  • Kurita K, Burgess SM, Sakai N (2004). Transgenic zebrafish produced by retroviral infection of in vitro cultured sperm. Proc. Natl. Acad. Sci. USA 101, 1263-1267.
    （レトロウイルスを感染させた精原細胞からトランスジェニックを作出した論文）
  • Kurita K, Sakai N (2004). Functionally distinctive testicular cell lines of zebrafish to support male germ cell development. Mol Reprod Dev 62, 430-438.
  • Matsumoto T, Yukawa W, Nozaki Y, Nakashige R, Shinya M, Makino S, Yagura M, Ikuta T, Imanishi T, Inoko H, Tamiya G, Gojobori T (2004).  Novel algorithm for automated genotyping of microsatellites.  Nucleic Acids Res 32, 6069-6077

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Review

  • 酒井則良（2007）ゼブラフィッシュにおける in vitro の精子形成. 蛋白質核酸酵素　52巻　16号、2124-2129.
  • Sakai N (2004). Genetically modified sperm in fish. ISB New Report, April 1-3 (Verginia Tech Press).

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著書

  • 酒井則良（2014）遺伝子が語る生命38億年の謎（国立遺伝学研究所編）　第19章生殖系幹細胞の謎　悠書館
  • 酒井則良（2013）遺伝子図鑑（国立遺伝学研究所「遺伝子図鑑」編集委員会編）第2章-6動物の生殖細胞と体細胞、第3章-3受精　悠書館
  • 新屋みのり、酒井則良（2009）身近な動物を使った実験（鈴木範男編著）第１巻第２章メダカ、ゼブラフィッシュ　三共出版
  • 村井耕二、酒井則良、山田雅保、安達卓、宅見薫雄（2008）発生生物学（基礎生物学テキストシリーズ５）化学同人.

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特許

  • 酒井則良、河崎敏弘（2014）魚体内での他家組織の継代維持法　出願番号：特願2014-163587
  • 高田達之、小山芳江、酒井則良（2011）モロコ細胞株、その製造方法および用途　出願番号：特願2011－129549

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2003年以前（酒井の研究業績）

Article

  • Sakai N (2002). Transmeiotic differentiation of zebrafish germ cells into functional sperm in culture. Development129, 3359-3365.
    （ゼブラフィッシュの精原細胞‐精子培養系を確立した論文）
  • Kobayashi T, Fushiki S, Sakai N, Hara A, Amano M, Aida K, Nakamura M, Nagahama Y (1998). Oogenesis and changes in the levels of reproductive hormones in triploid female rainbow trout. Fisheries Sci 64, 206-215.
  • Sakai N, Burgess S, Hopkins N (1997). Delayed in vitro fertilization of zebrafish eggs in Hank's saline containing bovine serum albumin. Mol Marine Biol Biotechnol 6, 84-87.
  • Sakai N, Suzuki N, Nagahama Y (1996). Distribution of the site of 17a,20b-dihydroxy- 4-pregnen-3-one production in the spermiating amago salmon (Oncorhynchus rhodurus) testis. Biomed Res 17, 473-477.
  • Sakai N, Suzuki N, Nagahama Y (1996). Involvement of cyclic AMP in the regulation of testicular steroidogenesis of amago salmon (Oncorhynchus rhodurus). Biomed Res 17, 479-486.
  • Sakai N, Tanaka M, Takahashi M, Fukada S, Mason JI, Nagahama Y (1994). Ovarian 3b-hydroxysteroid dehydrogenase/D5-4-isomerase of rainbow trout: its cDNA cloning and properties of the enzyme expressed in a mammalian cell. FEBS lett 350, 309-313.
  • Fukada S, Sakai N, Adachi S, Nagahama Y (1994). Steroidogenesis in the ovarian follicle of madaka (Oryzias latipes, a daily spawner) during oocyte maturation. Dev Growth Differ 36, 81-88.
  • Sakai N, Tanaka M, Takahashi M, Adachi S, Nagahama Y (1993). Isolation and expression of rainbow trout (Oncorhynchus mykiss) ovarian cDNA encoding 3b-hydroxysteroid dehydrogenase/D5-4-isomerase. Fish Physiol Biochem 11, 273-279.
  • Nagahama Y, Yoshikuni M, Yamashita M, Sakai N, Tanaka M (1993). Molecular endocrinology of oocyte growth and maturation in fish. Fish Physiol Biochem 11, 3-14.
  • Takahashi M, Tanaka M, Sakai N, Adachi S, Miller WL, Nagahama Y (1993). Rainbow trout ovarian cholesterol side-chain cleavage cytochrome P450 (P450scc): cDNA cloning and mRNA expression during oogenesis. FEBS Lett 319, 45-48.
  • Kobayashi T, Sakai N, Adachi S, Asahina K, Iwasawa H, Nagahama Y (1993). 17a,20a-Dihydroxy-4-pregnen-3-one is the naturally occuring spermiation-inducing hormone in the testis of a frog, Rana nigromaculata. Endocrinol 133, 321-327. 
  • Kobayashi T, Sakai N, Fushiki S, Nagahama Y, Amano M, Aida K (1993). Testicular development and changes in the levels of reproductive hormones in triploid male rainbow trout. Nippon Suisan Gakkaishi 59, 981-989 (in Japanese).
  • Iwamatsu T, Toya Y, Sakai N, Terada Y, Nagata R, Nagahama Y (1993). Effect of 5-hydroxytryptamine on steroidogenesis and oocyte maturation in pre-ovulatory follicles of the medaka Oryzias latipes. Dev Growth Differ 35, 625-630.
  • Sakai N, Tanaka M, Adachi S, Miller WL, Nagahama Y (1992). Rainbow trout cytochrome P-450c17 (17a-hydroxylase/17,20-lyase): cDNA cloning, enzymatic properties and temporal pattern of ovarian P-450c17 mRNA expression during oogenesis. FEBS Lett 301, 60-64.
  • Hirai T, Yamashita M, Yoshikuni M, Tokumoto T, Kajiura H, Sakai N, Nagahama Y (1992). Isolation and characterization of goldfish cdk2, a cognate variant of the cell cycle regulator cdc2. Dev Biol 152, 113-120.
  • Nagahama Y, Matsuhisa A, Iwamatsu T, Sakai N, Fukada S (1991). A mechanism for the action of pregnant mare serum gonadotropin on aromatase activity in the ovarian follicle of the medaka, Oryzias latipes. J Exp Zool 259, 53-58.
  • Takahashi SY, Iwamatsu T, Sakai N, Onitake K (1991). Phosphorylation of follicle proteins from the teleost, Oryzias latipes, in the action of gonadotropin and forskolin. Biomed Res 12, 231-239.
  • Iwamatsu T, Oshima E, Sakai N (1990). Steroid production and morphology of cultured ovarian follicles of the medaka. Bull Aichi Univ Edu Vol. XXXVIV, 69-78 (in Japanese).
  • Sakai N, Ueda H, Suzuki N, Nagahama Y (1989). Steroid production by amago salmon (Oncorhynchus rhodurus) testes at different developmental stages. Gen Comp Endocrinol 75, 231-240.
  • Sakai N, Ueda H, Suzuki N, Nagahama Y (1989). Involvement of sperm in the production of 17a,20b-dihydroxy-4-pregnen-3-one in the testis of spermiating rainbow trout, Salmo gairdneri. Biomed Res 10, 131-138.
  • Sakai N, Iwamatsu T, Yamauchi K, Nagahama Y (1988). Influence of follicular development on steroid production in the medaka (Oryzias latipes) ovarian follicle in response to exogenous substrates. Gen Comp Endocrinol 71, 516-523.
  • Iwamatsu T, Ohta T, Oshima E, Sakai N (1988). Oogenesis in the medaka Oryzias latipes -stages of oocyte development. Zool Sci 5, 353-373.
  • Sakai N, Iwamatsu T, Yamauchi K, Nagahama N (1987). Development of the steroidogenic capacity of medaka (Oryzias latipes) ovarian follicles during vitellogenesis and oocyte maturation. Gen Comp Endocrinol 66, 333-342.  
  • Iwamatsu T, Takahashi S, Sakai N, Nagahama Y, Onitake K (1987). Induction and inhibition of in vitro oocyte maturation and production of steroids in fish follicles by forskolin. J Exp Zool 241, 101-111.
  • Iwamatsu T, Takahashi S, Sakai N, Asai K (1987). Inductive and inhibitory actions of a low molecular weight serum factor on in vitro maturation of oocytes of the medaka. Biomed Res 8, 313-322.

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Review

  • 酒井則良（2003）精子ベクターでトランスジェニックフィッシュを作出する. 化学と生物　第41巻　第4号、264-269.

著書

  • 長濱嘉孝、酒井則良、深田幸子（1990）メダカの生物学（江上信雄、山上健次郎、嶋昭紘編）第４章卵細胞の成熟.　東京大学出版会、pp 46-58.

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